Coding

Part:BBa_K3606812

Designed by: Mingwei Li, Gaochen Zhang   Group: iGEM20_Fudan   (2020-10-24)


mcbG

Usage and Biology:

McbG is responsible for immunity against MccB17.

Design:

Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbG into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbG expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbG product was produced, yet the result of colony PCR turns out as false positive.

Results:

We've constructed the plasmid for mcbG expression, yet the results of colony PCR is false positive thus further experiment is needed for the fundamental validation of the antimicrobial peptide(mccb17) expression. T--Fudan--Poster_Mcb-gels.jpg Figure2. gel map for mcbA, mcbC, mcbE and mcbF

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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